R2*-relaxometry can be used to non-invasively detect hepatic iron overload. However, most previous studies included patients with very high iron content. We sought to investigate if relaxometry reliably can detect lower levels of hepatic siderosis. R2* was therefore measured in patients with suspected chronic liver diseases of varying etiologies. We compared the relaxation rates to histological semiquantitative assessment as well as total liver iron content using mass spectrometry. There was good correlation between R2* and liver iron content. We also showed that R2*-relaxometry is better than histology when detecting slight iron overload.