Abstract #3167

A real-time metabolic investigation of the effect of hypothermia on hypoxic ischemia during mouse brain development using hyperpolarized 13C

Yiran Chen¹, Alkisti Mikrogeorgiou², Robert Bok¹, Subramaniam Sukumar¹, R Ann Sheldon^2,3, A James Barkovich^1,3, Donna M Ferriero^2,3, and Duan Xu¹

¹Department of Radiology and Biomedical Imaging, University of California San Francisco, San Francisco, CA, United States, ²Department of Neurology, University of California San Francisco, San Francisco, CA, United States, ³Department of Pediatrics, University of California San Francisco, San Francisco, CA, United States

In this study, we applied dynamic nuclear polarization (DNP) technique to investigate C1 labeled 13C pyruvate to lactate conversion to study the effect of hypothermia treatment on hypoxic ischemia (HI) injured neonatal mouse brains during development. Our results showed that lower pyruvate delivery to the injured hemisphere in comparison to the non-injured hemisphere at the day of injury (P10) for all subjects, and difference narrows as the brain matures. There were different individual responses to the lactate to pyruvate ratio between two hemispheres. With this technique, we are able to examine individual responses to treatment during brain development.

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