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Abstract #3556

A perfused heart system to simulate first pass observation of rat cardiac metabolism with hyperpolarized [1-¹³C]pyruvate and determination of LDH flux using selective excitation

Gal Sapir1, Talia Harris1, Assad Azar1, Atara Nardi-Schreiber1, Ayelet Gamliel1, Jacob Sosna1, Moshe J. Gomori1, and Rachel Katz-Brull1

1Radiology, Hadassah-Hebrew University Medical Center, Jerusalem, Israel

Aberrant cardiac metabolism is linked to major health issues in the Western world including diabetes and heart failure. New tools are needed to investigate these conditions and to allow better diagnosis. In this work we used dissolution dynamic nuclear polarization NMR spectroscopy (dDNP-NMR) to investigate [1-13C]pyruvate metabolism in the isolated rat heart. A perfusion system simulating in vivo first-pass hemodynamics was used to measure the enzymatic flux through lactate dehydrogenase (LDH) using product selective excitation. LDH flux was found to be 52 ± 8 nmol lactate/s/g wet weight (n=3).

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