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Abstract #3674

Optimized Fluorine-19 MRI Quantification and Characterization of Inflammation in Atherosclerotic Plaques in Mice at 3T

Emeline Darçot1, Roberto Colotti1, Jérôme Yerly1,2, Maxime Pellegrin3, Anne Wilson4, Stefanie Siegert4, Matthias Stuber1,2, and Ruud B. van Heeswijk1,2

1Department of Radiology, Lausanne University Hospital (CHUV) and University of Lausanne (UNIL), Lausanne, Switzerland, 2Center for Biomedical Imaging (CIBM), Lausanne, Switzerland, 3Division of Angiology, Lausanne University Hospital (CHUV), Lausanne, Switzerland, 4Department of Fundamental Oncology, University of Lausanne (UNIL), Lausanne, Switzerland

Establishing a direct quantification and monitoring of inflammation in diseases such as atherosclerosis in a clinical setting is one of the main goals of fluorine-19 (19F) MRI of perfluorocarbons (PFCs). To this end, with 19F imaging and a denoising algorithm, we demonstrated the feasibility of quantitative 19F MRI in small inflammation sites such as atherosclerotic plaques in mice at 3T (average PFCs concentration per plaque=0.49±0.10mM; average plaque volume=2.8±1.1mm3). In a second step, with imaging flow cytometry, we characterized and visualized the PFC-incorporating immune cell populations involved in the inflammation process, which were mainly dendritic cells, macrophages and neutrophils (ratio 9:1:1).

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