Magnetic resonance-acoustic radiation force imaging (MR-ARFI) pulse sequences permit localization and targeting during focused ultrasound (FUS) therapy. MR-ARFI uses motion-encoding gradients (MEGs) to visualize the tissue displacement caused by the acoustic beam’s radiation force. However, a priori knowledge of the acoustic beam’s position and orientation in space is critical for MR-ARFI so that the MEGs can be placed in the proper orientation. We used an optical tracking system to inform the geometry of MR-ARFI acquisitions. The proposed methods will be used to guide ongoing experiments that use MR-ARFI to produce acoustic beam maps for targeting ultrasound neuromodulation in real-time.