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Abstract #5118

GluCEST MRI: Reproducibility, background contribution and source of glutamate changes in the MPTP mouse model of Parkinson’s disease

Puneet Bagga1, Stephen Pickup1, Dan Martinez2, Rachelle Crescenzi1, Ari Borthakur1, Gaurav Verma1, Joel Greenberg3, John Detre3, Hari Hariharan1, and Ravinder Reddy1

1Department of Radiology, University of Pennsylvania, Philadelphia, PA, United States, 2Children's Hospital of Philadelphia, Philadelphia, PA, United States, 3Department of Neurology, University of Pennsylvania, Philadelphia, PA, United States

Glutamate Chemical Exchange Saturation Transfer (GluCEST) MRI provides indirect detection of glutamate in vivo by measuring the exchange of glutamate amine protons with bulk water. The GluCEST contrast is potentially contaminated by a contribution of other metabolites exhibiting proton chemical exchange. We evaluated the reproducibility and background contamination to the GluCEST and source of the GluCEST changes in MPTP mouse model. Approximately 28% of GluCEST contrast appears to be derived from sources other than glutamate that are also not detectable by MRS. Glial proliferation caused by neuroinflammation was found to be the cause of elevated glutamate in mice exposed to MPTP.

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