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Abstract #5129

Non-invasive Detection of NADH+NAD+ in Human Muscle Using 31P MR Spectroscopy at 3T

Rajakumar Nagarajan1, Miles Bartlett2, Kwan-Jin Jung1, Jane A Kent2, and Nagendra Yadava3,4,5

1Human Magnetic Resonance Center, Institute for Applied Life Sciences, University of Massachusetts, Amherst, MA, United States, 2Department of Kinesiology, UMass, Amherst, MA, United States, 3Department of Biology, UMass, Amherst, MA, United States, 4Pioneer Valley Life Sciences Institute (PVLSI), Springfiled, MA, United States, 5Department of Medicine, University of Massachusetts Medical School (UMMS)-Baystate Regional Campus, Springfiled, MA, United States

NAD+ and NADH act as coenzymes in metabolic reactions. The reduction of NAD+ to NADH is linked with generation of ATP through glycolysis and oxidative phosphorylation. The consumption of NAD+ by various signaling proteins regulates protein modification, cell fate and survival. Therefore, NAD+ and NADH measurements have the potential to inform about tissue energetics and health. Recently, some investigators have suggested that NADH and NAD+ may be detected in human muscle using 31-phosphorus MRS. However, the utility and reliability of this measure is not clear. The goals of this project were to 1) determine whether the NADH+NAD peak can be resolved in human skeletal muscle at 3T, 2) compare peak resolution with and without a decoupling technique, and 3) evaluate the reliability of this measure. Interpretation of these data and their potential for studying alterations in NAD+ and NADH homeostasis in human muscle remain to be determined.

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