This study aimed to validate T2- and susceptibility-based cerebral venous oxygen saturation (SvO2) measurements with the clinical standard, jugular vein catheterization. T2-relaxation-under-tagging (TRUST) and susceptibility-based oximetry (SBO) were performed on two healthy subjects with jugular catheterization and eleven subjects without catheterization under baseline, hypoxia and hypercapnia. TRUST tightly agreed with the jugular reference under hypercapnia but significantly underestimated SvO2 under baseline and hypoxia. Bias between SBO and the reference was independent on the physiological state. A proportional bias was observed comparing TRUST and SBO. The results suggested caution for inter-subject comparison of absolute SvO2 measurements using either TRUST or SBO.