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Abstract #0722

Metabolism of hyperpolarized [1-13C]pyruvate in awake, isoflurane and urethane anesthetized rat brain

Viivi Hyppönen1, Petteri Stenroos1, Riikka Nivajärvi1, Jan-Henrik Ardenkjær-Larsen2, Olli Gröhn1, Jaakko Paasonen1, and Mikko Kettunen1

1Biomedical Imaging Unit, Department of Neurobiology, A. I. Virtanen Institute, University of Eastern Finland, Kuopio, Finland, 2Technical University of Denmark, Kongens Lyngby, Denmark

Pre-clinical MRI/MRS routinely uses anesthesia, which alters hemodynamics and metabolism. Here, we used hyperpolarised [1-13C]pyruvate to compare brain metabolism under isoflurane or urethane anesthesia and in awake rats. Spectroscopic [1-13C]pyruvate time courses were measured in sequence in awake, isoflurane and urethane anesthetized rats. Bicarbonate- and lactate-to-total carbon ratios decreased in order from awake animals to urethane to isoflurane anesthetized animals. No change was observed in bicarbonate-to-lactate ratio between the groups, however. The study shows dDNP experiments can be performed in awake rats thus avoiding issues related to anesthesia. However, the ratios between intracellular metabolites did not alter in awake rats.

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