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Abstract #1146

Whole-brain functional magnetic resonance imaging of serotonergic neuron optogenetic activation

Madalena S. Fonseca1, Zachary F Mainen1, and Noam Shemesh1

1Champalimaud Research, Champalimaud Centre for the Unknown, Lisbon, Portugal

Serotonin (5-HT) has been implicated in a variety of brain processes and is a main target of psychoactive drugs, yet we know little about where and how 5-HT acts to ultimately shape behaviour. Here, we optogenetically stimulated serotonergic neurons in the mouse dorsal raphe nucleus (DRN), and mapped the ensuing downstream activity using BOLD fMRI. Furthermore, by pairing 5-HT stimulation with sensory stimuli, we revealed how 5-HT stimulation affects sensory-evoked activity. Our findings highlight the feasibility of using opto-fMRI, with and without concurrent manipulations, to study 5-HT modulation on downstream circuitry in the global brain in-vivo.

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