The impact of renal perfusion on magnetization transfer imaging (MTI) was evaluated on seven pigs that underwent consecutive MRI at baseline, 50%, 75%, and 100% acute renal artery stenosis as well as reperfusion. Renal perfusion, R2*, apparent diffusion coefficient (ADC), stiffness, and MT ratio (MTR) were measured. During graded ischemia, decline in renal perfusion was accompanied with elevated R2*, decreased ADC and stiffness. In contrast, no change was observed in the MTR. In conclusion, MTI is insensitive to decrease in renal perfusion and may offer a relatively reliable technique for measurement of renal structural changes.