Meeting Banner
Abstract #2260

Effects of Anaesthetic Duration and Time of Day on Metabolite Levels in Long Evans Rat

Wendy Oakden1 and Greg J Stanisz1,2,3

1Physical Sciences Platform, Sunnybrook Research Institute, Toronto, ON, Canada, 2Medical Biophysics, University of Toronto, Toronto, ON, Canada, 3Department of Neurosurgery and Pediatric Neurosurgery, Medical University of Lublin, Lublin, Poland

The type and dose of anaesthesia affects both cerebral blood flow and metabolism. For experiments which involve measuring small changes in neuro-chemical levels either between groups, or following treatment, any differences in experimental conditions which increase the amount of variability can either mask or alter the results. Using 1H MRS, metabolites were quantified hourly in hippocampus and thalamus over the course of 5 hours under light isoflurane anaesthetic, in rats exposed to either a normal or a reversed light cycle. Both anaesthesia duration and light cycle had statistically significant effects on some, but not all, metabolites.

This abstract and the presentation materials are available to members only; a login is required.

Join Here