The type and dose of anaesthesia affects both cerebral blood flow and metabolism. For experiments which involve measuring small changes in neuro-chemical levels either between groups, or following treatment, any differences in experimental conditions which increase the amount of variability can either mask or alter the results. Using 1H MRS, metabolites were quantified hourly in hippocampus and thalamus over the course of 5 hours under light isoflurane anaesthetic, in rats exposed to either a normal or a reversed light cycle. Both anaesthesia duration and light cycle had statistically significant effects on some, but not all, metabolites.
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