Meeting Banner
Abstract #2263

Indirect 1H-[13C] NMR Spectroscopy in Rodent Alzheimer’s Model

Steven Zhang1,2, Masoumeh Dehghani2,3, Jim Gourdon4, Chathura Kumaragamage5, and Jamie Near2,3

1Integrative Program in Neuroscience, McGill University, Montreal, QC, Canada, 2Brain Imaging Center, Douglas Mental Health Institute, Montreal, QC, Canada, 3Department of Psychiatry, McGill University, Montreal, QC, Canada, 4Comparative Medicine & Animal Resources Centre, McGill University, Montreal, QC, Canada, 5Yale University, New Haven, CT, United States

Alzheimer’s disease (AD) is a neurodegenerative disorder commonly associated with neuronal metabolic deficits. In this study, we employ an indirect 1H-[13C] MRS approach to investigate changes in glucose metabolism between wild type and transgenic Alzheimer rats. This method can be used to follow the labeling of downstream metabolites after [1-6,13C2] glucose infusion and allows for quantitative measurements of the rate of 13C-label transfer from glucose into glutamate and glutamine in the brain. Preliminary comparison of 13C fractional enrichment time courses suggest that transgenic rats exhibit slower 13C metabolite labeling, indicative of a lowered glucose metabolic rate in AD pathology.

This abstract and the presentation materials are available to members only; a login is required.

Join Here