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Abstract #2295

Ex vivo quantification of hepatic fatty acid in mice fed with western-diet using magnetic resonance spectroscopy at 9.4 T

Aline Xavier1, Flavia Zacconi 2, Daniel Cabrera 3, Marco Arese3, and Marcelo Andia1

1Biomedical Imaging Center, Pontificia Universidad Católica de Chile, Santiago, Chile, 2Faculty of Chemistry, Pontificia Universidad Católica de Chile, Santiago, Chile, 3Gastroenterology Department, Pontificia Universidad Católica de Chile, Santiago, Chile

The idea of defining a biomarker to assess the progression of Nonalcoholic Fatty Liver Disease (NAFLD) by using Magnetic Resonance Spectroscopy (MRS) emerges due to the need to find a way to replace biopsy with a non-invasive method. The purpose of this study is to investigate the composition of the liver fatty acids based in the metabolite signals in MRS in NAFLD mice fed with a western-diet at 3 time-point during the progression of the disease. Our findings showed significant changes in the diallylic (2.8 ppm), olefinic (5.3ppm), allilic (2.0 ppm) and bulk methylene (1.3 ppm) peaks during the progression of the NAFLD by using high-resolution MRS.

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