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Abstract #2861

The Initial Tumor Microenvironment in Cerebral Patient-Derived Glioma Xenografts Affects Their Phenotypical Presentation

James R Ewing1,2, Ana deCarvalho2, Brent Griffith3, Stephen Brown4, George Divine5, Susan Irtenkauf2, Robert A Knight1, Ian Y Lee2, Swayamprava Panda1, Glauber Cabral1, and Taverekere N Nagaraja2

1Neurology, Henry Ford Health System, Detroit, MI, United States, 2Neurosurgery, Henry Ford Health System, Detroit, MI, United States, 3Radiology, Henry Ford Health System, Detroit, MI, United States, 4Radiation Oncology, Henry Ford Health System, Detroit, MI, United States, 5Public Health Sciences, Henry Ford Health System, Detroit, MI, United States

Patient-derived xenografts (PDXs) of human gliomas in murine models are unmatched in representing the molecular heterogeneity of the disease, but typically do not present with MRI contrast, and thus are not phenotypical. This may pose a limit to the assessment of trial therapies. PDX preparations were tweaked by co-injecting Matrigel with neurospheres. In one of the two cell lines studied, Matrigel promoted the formation of brain tumors whose genetic composition was that of the original human GBMs and whose radiologic appearance on MRI was similar to that seen in humans. In the second cell line, no BBB breakdown occurred.

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