This study aims to use the graph-based theoretical analysis to investigate the topological properties of the functional brain connectome in amyotrophic lateral sclerosis(ALS).151 healthy controls and 101 patients underwent a rs-fMRI scan. Compared with controls, brain networks of ALS patients were characterized by decreased global efficiency and the characteristic path length increased. Based on these perspectives, the ALS group exhibited “regularization small-worldization”. A network with 10 nodes and 16 edges was identified that was significantly altered in default mode network (DMN) regions. This study may provide novel insights into the pathophysiological mechanisms underlying psychiatric disorders from a connectomic perspective.