Cardiac PCr/ATP ratios measured by 31P MRS are a measure of the biochemical “energy reserve” in the heart. They decrease early in the progression of a wide range of diseases, making them a valuable biomarker. Logistically, it is often important for a long-running study to be able to compare data acquired on different MRI scanners. In this study, we show that comparable results may be acquired with 3T Prisma and 3T Trio scanners by scanning 10 healthy volunteers with 31P-CSI and 31P-DRESS on both systems. It is legitimate to pool 3T Prisma and 3T Trio data for clinical studies.