The purpose of the study was to propose a multiple-echo-time (TE) in vivo proton magnetic resonance spectroscopy (MRS) for a concurrent quantification and T2 measurement of glutamate. Point-resolved spectroscopy with eight TE values was used. The validity of selected TEs for T2 estimation of glutamate was verified by the spectral analysis. Comparison results showed that the reliability of the proposed method was comparable to that of typically used MRS techniques for quantification and T2 estimation, respectively. The proposed method can concurrently measure concentration and T2 of glutamate and effectively investigate brain metabolism and microenvironment in an acceptable scan time.