The feasibility of acquiring hyperpolarized 13C images from human brains following injections of HP [1-13C]pyruvate solution has been recently demonstrated. In this study, following each dynamic 3D volume 13C imaging acquisition (5 s temporal resolution), 13C spectroscopic data were acquired from a large slab through the brain. 13C pyruvate, lactate, alanine and bicarbonate were observed in all volunteer subjects. 13C aspartate was also detect in some of the subjects. Good inter-subject consistency in metabolite ratios as well as potential age related trend were observed in these data.