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Abstract #4319

Per-deuteration does not affect metabolic rates of hyperpolarized pyruvate in the isolated heart

Alexander Funk1, Chalermchai Khemtong1, Nesmine Maptue1, Dean Sherry1,2, and Craig Malloy1,3

1UT Southwestern Medical Center, Dallas, TX, United States, 2UT Dallas, Richardson, TX, United States, 3Veterans Affairs North Texas Healthcare System, Dallas, TX, United States

The effects of deuteration in pyruvate on conversion to alanine and lactate, and flux into the TCA cycle were investigated by 13C NMR of tissue extracts from isolated hearts and studies of hyperpolarized [1-13C1, 3,3,3-2H3]pyruvate. No kinetic isotope effects were noted, but substantial exchange of water protons with deuterium was observed in alanine but not lactate or glutamate. Deuteration of pyruvate had little effect on metabolism in heart tissue, and the data suggests that formation of a Schiff base in alanine transaminase may occur without net interconversion of pyruvate and alanine.

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