19F MR spectroscopy and imaging represent important tools for the development of new MR contrast agents and pharmaceutics. Until now, there are only a few published data that described the influence of temperature changes to the 19F chemical shifts in aqueous solutions. Temperature coefficients up to ~ 8.7Hz/K were determined. Thermoresponsive agents are of high interest in, e.g. hyperthermia studies. Changes in signal intensity and chemical shift give information of the local temperature. Here, we present novel MR spectroscopic and imaging data, which describe the 19F MR signal temperature dependency of different fluorinated organic substrates in isotonic saline solution and their temperature calculation methods.