Abstract #4375

Real-time Metabolic Investigations of Living Cells by NMR – First Experiences

Damian Hertig^1,2,3, Andrea Debora Felser¹, Gaëlle Diserens², Sandra Kurth¹, Jean-Marc Nuoffer^1,4, and Peter Vermathen²

¹Departments DOLS, University Hospital of Bern, Switzerland, Institute of Clinical Chemistry, University Hospital Bern, Switzerland, Bern, Switzerland, ²Departments of Clinical Research and Radiology, University of Bern, Switzerland, Magnetic Resonance Spectroscopy and Methodology - Department of Clinical Research - University Bern/Switzerland, Bern, Switzerland, ³Graduate School for Cellular and Biomedical Sciences, University of Bern, Switzerland, Bern, Switzerland, ⁴Department of Pediatric Endocrinology, Diabetology and Metabolism, University Children’s Hospital of Bern, Switzerland, Bern, Switzerland

NMR approaches using flow systems for real-time spectroscopy of living cells were explored as early as the 80s but did not become routine due to different technical obstacles. We show the technical feasibility of real-time metabolic monitoring in 3D culture inside our established NMR bioreactor using the commercially available InsightCell^TM (Bruker) tool, which supposedly eases bioreactor setup. Human skin fibroblasts showed reproducible glucose and O₂ measurements in stop-and-go experiments over 12 hours. Chemical interventions on aerobic energy production pathways showed fast response by increasing rate of anaerobic glycolysis, which could be reversed by subsequent inhibition of glycolytic pathway.

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