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Abstract #4375

Real-time Metabolic Investigations of Living Cells by NMR – First Experiences

Damian Hertig1,2,3, Andrea Debora Felser1, Gaëlle Diserens2, Sandra Kurth1, Jean-Marc Nuoffer1,4, and Peter Vermathen2

1Departments DOLS, University Hospital of Bern, Switzerland, Institute of Clinical Chemistry, University Hospital Bern, Switzerland, Bern, Switzerland, 2Departments of Clinical Research and Radiology, University of Bern, Switzerland, Magnetic Resonance Spectroscopy and Methodology - Department of Clinical Research - University Bern/Switzerland, Bern, Switzerland, 3Graduate School for Cellular and Biomedical Sciences, University of Bern, Switzerland, Bern, Switzerland, 4Department of Pediatric Endocrinology, Diabetology and Metabolism, University Children’s Hospital of Bern, Switzerland, Bern, Switzerland

NMR approaches using flow systems for real-time spectroscopy of living cells were explored as early as the 80s but did not become routine due to different technical obstacles. We show the technical feasibility of real-time metabolic monitoring in 3D culture inside our established NMR bioreactor using the commercially available InsightCellTM (Bruker) tool, which supposedly eases bioreactor setup. Human skin fibroblasts showed reproducible glucose and O2 measurements in stop-and-go experiments over 12 hours. Chemical interventions on aerobic energy production pathways showed fast response by increasing rate of anaerobic glycolysis, which could be reversed by subsequent inhibition of glycolytic pathway.

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