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Abstract #5015

Inaccuracy in Estimated CEST Contrast After Extrapolation from Two-Pool Quantitative MT Fitting

Wilfred W Lam1, Ryan T Oglesby1,2, and Greg J Stanisz1,2,3

1Physical Sciences, Sunnybrook Research Institute, Toronto, ON, Canada, 2Medical Biophysics, University of Toronto, Toronto, ON, Canada, 3Neurosurgery and Paediatric Neurosurgery, Medical University of Lublin, Lublin, Poland

A recently introduced technique has been used to remove the semisolid macromolecular magnetization transfer (MT) contribution from Z-spectra in order to isolate the contrast from chemical exchange saturation transfer (CEST) and the relayed nuclear Overhauser effect (rNOE). This is usually performed by first fitting Z-spectra acquired with high amplitude RF saturation with a two-pool quantitative MT model and then extrapolating an MT-only Z-spectrum to a lower saturation B1 and subtracting from Z-spectra acquired with the same B1. We aim to show that this two-step pipeline introduces inaccuracies into the extrapolation and describe them.

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