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Abstract #0273

In vivo fluorine-19 MRI of intracellular oximetry response to chimeric antigen receptor T cell therapy against glioma

Fanny Chapelin1, Wenlian Zhu2, Benjamin Leach2, Hideho Okada3, and Eric Ahrens2
1Biomedical Engineering, University of Kentucky, Lexington, KY, United States, 2Radiology, University of California San Diego, La Jolla, CA, United States, 3Neurological Surgery, University of California San Francisco, San Francisco, CA, United States

We explore the temporal dynamics of tumor and T cell intracellular partial pressure of oxygen (pO2) in a murine flank glioma model receiving chimeric antigen receptor (CAR) T cell therapy. Tumor cells or T cells are intracellularly labeled with perfluorocarbon nanoemulsion prior to injection. 19F MRI relaxation rate measurements are used to elucidate intracellular pO2 in vivo. The tumor pO2 peaks at 3 days post-infusion, commensurate with CAR T cell infiltration and tumor cell killing. Moreover, the absolute 19F signal scales with tumor burden. Overall, 19F pO2 MRI measurements can assay cell-mediated apoptosis and provide insight into effector cell function.

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