Abstract #0382

Multi-sample measurement of pyruvate/lactate flux in melanoma cells using an HP micromagnetic resonance spectrometer and D2O solvation

Hannah J. Lees¹, Micaela Millan¹, Fayyaz Ahamed², Roozbeh Eskandari¹, Kristin L. Granlund¹, Sangmoo Jeong¹, and Kayvan R. Keshari¹

¹Memorial Sloan Kettering Cancer Center, NEW YORK, NY, United States, ²University of California, Berkeley, Berkeley, CA, United States

The pyruvate-lactate flux, k_PL, shows promise as a biomarker of cancer presence and aggressiveness, and assessment of k_PL in patient-derived cells may be a useful tool to assess treatment response for advanced personalized medicine. Here we present a novel experimental protocol for the real-time measurement of pyruvate-lactate metabolic flux in multiple mass-limited cell suspension samples using a single dissolution, thereby increasing efficiency and providing greater control of the methodological variability associated with HP experiments. We then applied this protocol to the measurement of pyruvate-lactate flux in melanoma cells for the assessment of treatment response to BRAF inhibition.

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