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Abstract #0382

Multi-sample measurement of pyruvate/lactate flux in melanoma cells using an HP micromagnetic resonance spectrometer and D2O solvation

Hannah J. Lees1, Micaela Millan1, Fayyaz Ahamed2, Roozbeh Eskandari1, Kristin L. Granlund1, Sangmoo Jeong1, and Kayvan R. Keshari1
1Memorial Sloan Kettering Cancer Center, NEW YORK, NY, United States, 2University of California, Berkeley, Berkeley, CA, United States

The pyruvate-lactate flux, kPL, shows promise as a biomarker of cancer presence and aggressiveness, and assessment of kPL in patient-derived cells may be a useful tool to assess treatment response for advanced personalized medicine. Here we present a novel experimental protocol for the real-time measurement of pyruvate-lactate metabolic flux in multiple mass-limited cell suspension samples using a single dissolution, thereby increasing efficiency and providing greater control of the methodological variability associated with HP experiments. We then applied this protocol to the measurement of pyruvate-lactate flux in melanoma cells for the assessment of treatment response to BRAF inhibition.

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