Differentiation between glioblastomas (GBMs) and cerebral metastases based on MR structural images is often challenging due to the poor specificity. MRSI is a useful tool for mapping the metabolic fingerprints of tumors. In this study, we investigate the use of a high-resolution MRSI technique known as SPICE for differentiation between GBMs and cerebral metastases in 28 patients. Our results show the metabolic biomarkers are different between GBMs and metastases as well as in the enhancing ring and the core of neoplasms. SPICE potentially provides a non-invasive metabolic measure to differentiate GBMs from cerebral metastases with high resolution.