Isocitrate dehydrogenase (IDH) mutation highly affects the overall survival of gliomas. In addition to the IDH mutation, the tumor histologic grade might play a role in patient prognosis. The aim of this study was to assess the metabolic variations between different tumor grades within IDH mutant (IDH-mut) and IDH wild-type (IDH-wt) gliomas using proton MR spectroscopy. Higher glycine in glioblastoma (GBM) within IDH-mut, and lower Cr and mIns in GBM within IDH-wt were the only statistically significant differences. Our study indicated similar metabolic profiles of different grades within IDH mutational subgroups, supporting IDH as a better predictor of clinical outcome.