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Abstract #0497

The CLARITY procedure of lipid removal from brain tissue sample reveals the lipid-origin of MT contrast in CEST imaging experiment

Anna Orzylowska1, Tymoteusz Słowik2, Agata Chudzik1, Anna Pankowska3, Wilfred W Lam4, and Greg J Stanisz1,4,5
1Department of Neurosurgery and Paediatric Neurosurgery, Medical University of Lublin, Lublin, Poland, 2Center of Experimental Medicine, Medical University of Lublin, Lublin, Poland, 3Department of Radiography, Medical University of Lublin, Lublin, Poland, 4Physical Sciences, Sunnybrook Research Institute, Toronto, ON, Canada, 5Department of Medical Biophysics, University of Toronto, Toronto, ON, Canada

The study compares the differences between Z-spectra derived from CEST imaging of rat brain in vivo and after post-mortem CLARITY lipids removal procedure. The lipids removal nulled-out MT macromolecular-originating signal measured with B1 saturation amplitudes of 3 and 5 µT as compared to in vivo, and resulted in negligible MT contribution to CEST Z-spectra acquired with B1s of 0.5 and 0.75 µT, as opposite to living tissue, where the MT effect was significant. Our results showed that the macromolecular MT contribution into in vivo Z-spectra originates mostly from lipids, since the CLARITY technique removed the MT component from the spectrum.

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