The feasibility of T1, T2 and T2* in brain imaging and lesion quantification has been proved. However, studies about quantitative imaging seldom quantify T1, T2 and T2* together. This study proposed a three-dimensional (3D) simultaneous quantitative T1-T2-T2* mapping (SQUMA) for the whole brain. SQUMA sequence was composed of five dynamic scans using variable flip angles, variable T2 preparation duration and multi-echo acquisitions. SQUMA sequence showed excellent agreement with reference imaging in measuring T1, T2 and T2* values (R2=0.98, 0.84 and 0.90, respectively) and good to excellent repeatability in in-vivo studies. It is feasible to use SQUMA in clinical applications.
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