CSF flow has been most commonly evaluated using a gated 2D phase contrast (PC) acquisition at the cerebral aqueduct or foramen magnum. However, real-time acquisitions that allow for evaluation of changes in flow with the cardiac and respiratory may provide additional insight into CSF dynamics disorders. In this study we apply a real-time EPI based PC acquisition for imaging of intracranial CSF flow at multiple intracranial locations. Quantitative analyses are validated by comparison with a standard phase contrast acquisition. Frequency spectra analysis demonstrates dominant variations in CSF flow with the cardiac and respiratory cycles.