DWI can evaluate pregnancy-related dysfunctions, yet EPI’s sensitivity to motions and air/water/fat heterogeneities complicate these studies in preclinical settings. We have developed DWI methodologies based on SPatiotemporal ENcoding (SPEN) for overcoming these obstacles, delivering single-shot images at ≈100µm in-plane resolutions. These methods were used to monitor fetoplacental differences between naïve and knockout mice strains mimicking preeclampsia and IUGR. High definition ADC/DTI maps could resolve the placental layers (maternal, fetal, trophoblastic), umbilical cords, and various brain compartments in the developing fetuses. Daily monitoring also showed differences in the development of placental and fetal (e.g. brain) structures among normal and disease models.