We investigated whether deuterium MR spectroscopy using 2H-labeled glucose can be used to assess Krebs cycle metabolism in the in-vivo heart. Localized 2H spectra were acquired from a slice containing the whole heart and from a voxel containing only the left ventricle of the heart using a temporal resolution of ~12 min following a bolus injection of [6,6′-2H2]glucose. Single voxel spectra show the production of labelled Glutamate/Glutamine (Glx) ˜36 min after the administration of the glucose. The Glx concentration reflects Krebs cycle activity which holds potential to probe various metabolic states of the heart.