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Abstract #0691

Hyperpolarized [1-13C] Glycerate as Probe to Assess Glycolytic Activity in a Rat Model of Hepatocellular Carcinoma

Jun Chen1, Evan LaGue2, Junjie Li1, Edward Hackett1, Ian Corbin1, Kelvin Billingsley2, and Jae Mo Park3,4
1AIRC, UT Southwestern Medical Center at Dallas, Dallas, TX, United States, 2Chemistry and Biochemistry, California State University Fullerton, Fullerton, CA, United States, 3UT Southwestern Medical Center at Dallas, Dallas, TX, United States, 4Electrical Engineering, University of Texas at Dallas, Richardson, TX, United States

Hyperpolarized [1-13C] glycerate was used to study the in vivo glycolytic activity in a rat model of hepatocellular carcinoma (HCC). Carbon-13 labeled glycolytic intermediate phosphorenolpyruvate (PEP) was detected in the tumor in addition to pyruvate and lactate peaks. The in vivo results were confirmed by high resolution 13C NMR spectra of tissue extracts, after steady-state infusion of [2,3-13C2] glycerate. The results illustrate the potential of [1-13C] glycerate as a metabolic probe for assessing glycolytic flux.

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