The purpose was to explore if T2’mapping can assess renal oxygen in the ischemia-reperfusion injury (IRI). IRI models were established according to different ischemia time, followed by injection of furosemide 24 hours after IRI and consecutive MRI scans. Quantitative scores of oxygen were acquired with the hypoxic probe. We found that R2’ values of the inner and outer medulla were statistically significant. R2’ value of the outer medulla was highly correlated with oxygen scores. T2’mapping could serve as a quantitative biomarker to assess the renal oxygen and monitor the treatment in patients with IRI.