Although tractography research was focused primarily on the human brain, tractography was integrated into animal models to benefit from various preclinical experiments. Accurate segmentation is required for proper connectome of animal models. The Allen mouse brain atlas can provide accurate coordinates and segmentation information to the mouse brain, but it is difficult to use because it is not MRI data. In this study, we use the ABA to accurately segment the mouse brain and examine tractography. In addition, various NEX are used to determine the changes in tractography caused by an increase in the SNR of the DTI.