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Abstract #1339

Simultaneous fMRI and mesoscopic Ca2+ imaging indicates spontaneous excitatory neural activity accounts for 1/3rd of the variance in BOLD signal

Evelyn MR Lake1, Xinxin Ge2, Xilin Shen1, Peter Herman1, Fahmeed Hyder1, Jessica A Cardin3, Michael J Higley3, Dustin Scheinost1, Xenophon Papademetris1, Michael C Crair2, and R Todd Constable1
1Radiology and Biomedical Imaging, Yale University, New Haven, CT, United States, 2Department of Neurobiology, Yale University, New Haven, CT, United States, 3Department of Neuroscience, Yale University, New Haven, CT, United States

We demonstrate longitudinal simultaneous whole-cortex Ca2+ imaging and fMRI in mice expressing GCaMP in one of five different cell types (excitatory, inhibitory, two interneuron subtypes, and astrocytes). The high SNR of our dual-imaging approach is shown by the indistinguishable Ca2+ responses to hind-paw or visual stimulation measured inside and outside the scanner. We optimize a spatially variable, three-parameter gamma-variant to investigate the transfer function between the BOLD and Ca2+ signals throughout the cortex. This approach is applied in functionally and anatomically defined ROIs. Results show that 1/3rd of the variance in BOLD is accounted for from spontaneous excitatory Ca2+ activity.

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