Abstract #1339

Simultaneous fMRI and mesoscopic Ca2+ imaging indicates spontaneous excitatory neural activity accounts for 1/3rd of the variance in BOLD signal

Evelyn MR Lake¹, Xinxin Ge², Xilin Shen¹, Peter Herman¹, Fahmeed Hyder¹, Jessica A Cardin³, Michael J Higley³, Dustin Scheinost¹, Xenophon Papademetris¹, Michael C Crair², and R Todd Constable¹

¹Radiology and Biomedical Imaging, Yale University, New Haven, CT, United States, ²Department of Neurobiology, Yale University, New Haven, CT, United States, ³Department of Neuroscience, Yale University, New Haven, CT, United States

We demonstrate longitudinal simultaneous whole-cortex Ca²⁺ imaging and fMRI in mice expressing GCaMP in one of five different cell types (excitatory, inhibitory, two interneuron subtypes, and astrocytes). The high SNR of our dual-imaging approach is shown by the indistinguishable Ca²⁺ responses to hind-paw or visual stimulation measured inside and outside the scanner. We optimize a spatially variable, three-parameter gamma-variant to investigate the transfer function between the BOLD and Ca²⁺ signals throughout the cortex. This approach is applied in functionally and anatomically defined ROIs. Results show that 1/3^rd of the variance in BOLD is accounted for from spontaneous excitatory Ca²⁺ activity.

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