Abstract #1355

Layer-specific optogenetic stimulation of motor cortex activates distinct brain-wide networks

Russell W Chan¹, Mazen Asaad², Bradley J Edelman¹, Hyun Joo Lee¹, Hillel Adesnik³, David Feinberg³, and Jin Hyung Lee^1,4,5,6

¹Neurology and Neurological Sciences, Stanford University, Stanford, CA, United States, ²Molecular and Cellular Physiology, Stanford University, Stanford, CA, United States, ³Helen Wills Neuroscience Institute, University of California, Berkeley, CA, United States, ⁴Bioengineering, Stanford University, Stanford, CA, United States, ⁵Neurosurgery, Stanford University, Stanford, CA, United States, ⁶Electrical Engineering, Stanford University, Stanford, CA, United States

The primary motor cortex (M1) consists of a stack of interconnected but distinct layers. However, knowledge of brain-wide circuit function of M1 layer-specific pathways is lacking. Here, we combined layer-specific Cre-driver mice, optogenetics, and fMRI with subsequent electrophysiological recordings to reveal distinct M1 layer-specific networks. All L2/3, L4, L5 and L6 stimulations evoked M1 fMRI responses, while only L5 and L6 evoked robust caudate putamen and ventrolateral thalamic nucleus responses. Subsequent LFP and spike recordings were in line with these fMRI results. Overall, our techniques and results could help investigate brain-wide layer-specific cortical circuit functions in development, aging and diseases.

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