The previous non-conventional MRI studies included both AQP4 positive and negative patients, thus yielding differing results, partly explained by cohort heterogeneity (i.e. antibodies status). No studies are available in MOG-Ab disease. We compared, using non-conventional imaging, brain MRI findings in 73 subjects with AQP4-ab, MOG-Ab disease, Multiple Sclerosis and in healthy controls. We found that MOG-Ab disease have a lower lesions load when compared to MS and AQP4-Ab disease and we demonstrated, for the first time, that significant changes occur in the deep grey matter while no significant changes were detected in the non-lesional white matter in MOG-Ab disease.