Myelin water imaging allows the assessment of relative myelination by estimating the myelin water fraction (MWF). This work investigated if the degree of MWF loss in multiple sclerosis is associated with T1 hypointense and isointense lesions. Within n = 1668 FLAIR lesions of n = 63 MS patients, 79% had a hypointense correlate, and 21% of the lesions had an isointense appearance on non-enhanced T1-weighted images. Significant differences were found for hypointense (MWFmean = 0.126) and isointense (MWFmean = 0.185) lesions. These results show varying degrees of myelin reduction.