Multiple Sclerosis lesions are pathologically heterogeneous. The Multi-component relaxation method (mcDESPOT) which allows the determination of relative myelination by estimating the myelin water fraction (MWF) could be valuable to capture the high variability of disease development. In a pilot study n=112 RRMS patients got MRI examination and MWF values were determined of white matter (WM) and individual lesions. The MWF of WM was 0.230 for healthy controls and 0.224 for RRMS patients. 2787 WM FLAIR lesions were examined. Patients with a higher degree of disability of EDSS ≥ 4 significantly have lower MWF values than patients with EDSS < 2.5.