Based on gradient echo (GRE) magnetic resonance phase data, quantitative susceptibility mapping (QSM) is a novel technology which allows the noninvasive assessment of magnetic tissue susceptibility distribution in hemodialysis (HD) patients. And iron deficiency in gray matter nuclei has been reported to lead to idiopathic restless legs syndrome (RLS) symptoms. In this study, we investigated the differences of iron deposition patterns between HD-RLS and HD-nRLS patients scanned at 3T. Compared with HD-nRLS patients, HD-RLS patients demonstrated reduced susceptibility in caudate nucleus and puteman. Hence, QSM can be used for HD-RLS diagnosis and intervention.