The MP2RAGE sequence provides fast volumetric T1 weighted MR imaging and offers the possibility to reconstruct quantitative T1 maps. Therefore, it is frequently applied for studying Multiple Sclerosis pathologies in recent years. The present work investigates and explains necessary protocol changes for applying MP2RAGE in fixated human brain acquisitions. Using the established protocol, it is shown that strong soft tissue contrast is reinstated and quantitative T1 values can be derived for normal appearing gray matter and lesions. Based on the feasibility of using long scan times, the isotropic resolution of the MP2RAGE could be even increased to 0.75mm.