Here we present an imaging technique for measuring regional gas uptake in a free-breathing mouse non-invasively. Gas- and dissolved-phase images were acquired simultaneously for a wide range of repetition times (13 ms up to 100 ms), and used to derive septal wall thickness measurements by fitting to a theoretical gas uptake model. The work shown here sets the groundwork for non-terminal longitudinal studies in rodent models of pulmonary radiation and fibrosis.
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