There is a growing interest in utilizing single-voxel $$$^1$$$H MR spectroscopy (MRS) for probing the triglyceride fatty acid composition by means of the number of double bounds (ndb) and number of methylene interrupted double bounds (nmidb) per triglyceride as well as the mean fatty acid carbon chain length (CL). The present study investigates the in vitro measurement agreement of fatty acid composition parameters between single-voxel multi-TE and short-TR multi-TI multi-TE (SHORTIE) STEAM spectroscopy and the gold standard gas chromatography-mass spectrometry (GC-MS) in human subcutaneous and visceral adipose tissue samples.
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