Non-invasive assessment of muscle quality is of relevance in chronic disease, where muscle deconditioning is prevalent. To quantify PCr kinetics, localised and non-localised 31P acquisitions were performed during ischemic plantar flexion exercise and recovery. Application of non-localised 31P acquisition resolved motion induced phasing issues and low temporal resolution apparent in 31P ISIS data acquisitions. This allowed quantitation of PCr kinetics in response to high intensity ischemic exercise, successfully providing a surrogate marker of muscle quality in fatigued CD patients. Application of this within-bore exercise protocol to fatigued CD patients and healthy control volunteers will provide insight into pathological fatigue mechanisms.