Expression of telomerase reverse transcriptase (TERT) is essential for tumor proliferation, including in primary glioblastomas. Inhibiting TERT expression is also a therapeutic strategy for glioblastomas. The goal of this study was to identify non-invasive magnetic resonance spectroscopy (MRS)-detectable metabolic biomarkers of TERT expression in glioblastoma cells. Our studies indicate that TERT expression is linked to redox, as indicated by higher 1H MRS-detectable reduced glutathione, and higher 13C MRS-detectable flux of glucose via the pentose phosphate pathway in TERT-expressing GBM cells. Hyperpolarized [U-13C, U-2H]glucose can monitor TERT expression in GBM cells, and may serve to noninvasively probe TERT expression in glioblastomas.