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Abstract #3010

Differentiating Between DBD and Normotensive Syngeneic Grafts in a Rat Lung Transplant Model Using HP [1-13C] Pyruvate MRI

Sarmad Siddiqui1, Federico Sertic1,2, Mehrdad Pourfathi1, Jon Snow1, Ian Duncan1, Stephen J Kadlecek1, and Rahim R. Rizi1
1Radiology, University of Pennsylvania, Philadelphia, PA, United States, 2Surgery, University of Pennsylvania, Philadelphia, PA, United States

Our previous study in a rat lung transplant model using HP [1-13C]-pyruvate MRI demonstrated that an increased lactate-to-pyruvate ratio (LtP) precedes lung rejection. However, most lungs in a clinical setting are obtained after donor brain death (DBD), which is associated with increased ischemia-reperfusion injury and risk of acute lung rejection. In this study, we transplanted DBD-lungs into syngeneic rats and compared the metabolism of DBD-donor lungs to our previous normotensive-donor model. The LtP of the DBD-graft is approximately two-fold higher than that of the other lungs, suggesting that HP [1-13C]-pyruvate imaging can be used to identify grafts with ischemia-reperfusion injury.

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