HP 13C-MRI with 13C-Pyruvate was performed in normal, healthy volunteers over four major body regions and organs, including head/brain, chest/heart, abdomen/liver, and pelvis/kidneys. Many individuals received multiple HP 13C injections and a variety of acquisition sequence. Pyruvate-to-lactate conversion rate measurements were reproducible across days and coil configurations. All injections and imaging were very well tolerated with no adverse effects observed in over 70 scans.