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Abstract #3023

Radical-free and metal-free hyperpolarized MRI using endogenous pyruvate analogues

Claudia C Zanella1, Andrea Capozzi1, Hikari A I Yoshihara1, Alice Radaelli1, Lionel P Arn2,3, Rolf Gruetter1, and Jessica A M Bastiaansen2,3
1Laboratory for Functional and Metabolic Imaging (LIFMET), EPFL, Lausanne, Switzerland, 2Department of Diagnostic and Interventional Radiology, CHUV, Lausanne, Switzerland, 3Department of Diagnostic and Interventional Radiology, UNIL, Lausanne, Switzerland

Using nonpersistent radicals generated by UV-irradiation of endogenous metabolite precursors for dissolution DNP avoids the need for radical filtration and may potentially lengthen the measurement window of hyperpolarized MRI measurements. Here, the endogenous pyruvate-analogues alpha-ketobutyrate and alpha-ketovalerate were proposed as nonpersistent radical precursors. Radical yields were characterized along with their performance as polarizing agents for in vitro and in vivo dDNP experiments. A 13C-glucose liquid state polarization of 26.4% was attained using alpha-ketobutyrate-derived radical, while pyruvate-derived radical yielded 21.7% (compared to 18.9% reported with the persistent trityl radical). Alpha-ketobutyrate was used to hyperpolarize [1-13C]butyrate and measure cardiac metabolism in vivo.

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