Nicotine addiction is still one of the most preventable causes of chronic illness and premature death, and cessation regardless of age has positive effects on health outcomes (WHO, 2015). As such, there is growing interest in developing novel therapies and diagnostic aids to treat and monitor the nicotine addiction across the lifespan. Glutamate weighted chemical exchange saturation transfer (GluCEST) imaging was used to spatially map the glutamate levels of dorsal anterior cingulate cortex (dACC). We observed lower GluCEST contrast in females in the dACC regardless of smoking status. No significant difference in GluCEST was observed between the smokers and non-smokers.